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The Benefit of Passion Fruit as an Anti-ulcerogenic Diet: Scientific Evidence by In-vitro and In-silico H+/K+ATPase Inhibitory Activity Assessment

Author(s):

Vineeth Ajaya Kumar, Mridula Ramkumar and S K Kanthlal*   Pages 1 - 9 ( 9 )

Abstract:


Background: H+/K+ ATPase a protein present in the gastric parietal cells is a better target for the prevention and treatment of gastric ulcer. Plant flavonoids have been reported to elicit anti-ulcer activity by inhibiting the proton pump as well as by antioxidant defense mechanism.

Methodology: Chloroform fraction of hydro-alcoholic extract of passion fruit was screened for proton pump inhibitory assay using goat parietal cell. In-silico computational docking studies were carried out using Glide program in order to validate the inhibitory action of selected constituents.

Report and Conclusion: The flavonoid rich fruit possess a promising radical scavenging activity against DPPH. 10.41μg/mL is sufficient to inhibit 50% of ATPase enzyme activity. A synergistic activity was also achieved by the fruit with sub-effective doses of lansoprazole. Fenton's oxidation induced by H2O2 was also blunted by the fruit extract. The in-vitro and in-silico findings indicated that, passion fruit can be a good dietary supplement for the prevention and management of ulcer.

Keywords:

Passion fruit, Ulcer, H+/K+ ATPase, flavonoids, oxidation

Affiliation:

Dept. of Pharmacology, Amrita School of Pharmacy, Amrita Vishwa Vidyapeetham, AIMS Health Science campus, Kochi, Dept. of Pharmacology, Amrita School of Pharmacy, Amrita Vishwa Vidyapeetham, AIMS Health Science campus, Kochi, Dept. of Pharmacology, Amrita School of Pharmacy, Amrita Vishwa Vidyapeetham, AIMS Health Science campus, Kochi



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