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Synthesis and Molecular Simulation Studies of Mandelic Acid Peptidomimetic Derivatives as Aminopeptidase N Inhibitors


Jiawei Chen, Qiaoli Lv and Guogang Tu*   Pages 1 - 8 ( 8 )


Background: The aminopeptidase N (APN) over-expressed in tumor cells plays a critical role in angiogenesis which makes the development of APN inhibitors an attractive strategy for cancer research.

Aims and Objectives: It is clinically significant to develop potential APN inhibitors for cancer treatment. Reported here are the design, synthesis, biological evaluation and molecular simulation of mandelic acid peptidomimetic derivatives as APN inhibitors.

Materials and Methods: Analysis of the binding mode of bestatin to APN lead to the design and synthesis of mandelic acid peptidomimetic derivatives. APN inhibitory activities in vitro were evaluated by spectrophotometric method. The binding mode of the target compounds with the APN binding site was studied relying on docking studies, molecular dynamics simulation experiments and binding energies calculation.

Results: The structures of target compounds were confirmed by IR, 1H-NMR and MS. All compounds exhibited different range of inhibitory ability with IC50 values lying in micromolar level. The compound 9m was found to be most potent as compared to other target derivatives. Molecular simulation revealed that ligand coordinating with the catalytic zinc ion is very important for inhibitory activities.

Conclusion: The compound 9m might represent a promising scaffold for the further development of novel anti-cancer drugs.


Enzyme inhibitors, Aminopeptidase N, Peptidomimetics, Molecular simulation


Department of Medicinal Chemistry, School of Pharmaceutical Science, NanChang University, 330006, Department of Science and Education, JiangXi Key Laboratory of Translational Cancer Research, JiangXi Cancer Hospital, 330029, Department of Medicinal Chemistry, School of Pharmaceutical Science, NanChang University, 330006

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